| Accrington Stanley | 0 - 1 | Chesterfield | 03/05/2025 16:00 |
| Bradford City | 1 - 0 | Fleetwood Town | 03/05/2025 16:00 |
| Bromley | 3 - 0 | Cheltenham Town | 03/05/2025 16:00 |
| Carlisle United | 2 - 2 | Salford City | 03/05/2025 16:00 |
| Colchester | 0 - 0 | Barrow AFC | 03/05/2025 16:00 |
| Crewe Alexandra | 0 - 1 | Walsall | 03/05/2025 16:00 |
| Morecambe | 1 - 2 | Harrogate Town | 03/05/2025 16:00 |
| Grimsby Town | 0 - 1 | AFC Wimbledon | 03/05/2025 16:00 |
| Newport County | 1 - 4 | Tranmere Rovers | 03/05/2025 16:00 |
| Notts County | 1 - 2 | Doncaster Rovers | 03/05/2025 16:00 |
| Port Vale | 0 - 1 | Gillingham | 03/05/2025 16:00 |
| Swindon Town | 0 - 0 | Milton Keynes | 03/05/2025 16:00 |
Les jeux d'argent et de hasard sont interdits aux mineurs (-18 ans)